Viability and Antifungal Activity of Lactobacillus plantarum HL-15 Oven Dried Culture during Storage
Tri Marwati
1
, Titiek F. Djaafar
1
, Evelyn E. Setiawan
2
, Tyas Utami
2
, Endang S. Rahayu
2
1
Postharvest Department, Assessment Institute for Agricultural Technology Yogyakarta, Indonesia
2
Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia
watipasca2@gmail.com
Abstract
Lactobacillus plantarum HL-15 has the ability to inhibit the growth of mycotoxin-producing fungi. To support the application of the use of these cultures, a study was carried out aimed at producing dry L. plantarum HL-15 and observing its stability during storage. Production of dry culture begins with fermentation of L. plantarum HL-15 then centrifugation is carried out to obtain pellets. Pellets were mixed with fillers (rice flour or tapioca) with a ratio of pellets: fillers (10%) = 1: 1 (v / v) and then dried. The drying machine used is an oven with a temperature of 45 ° C for 20 hours. The dry culture was packaged in aluminum foil and sealed and then stored at 4 ° C. The result showed that viable cells of oven-dried L. plantarum HL-15 with rice filler was <4log CFU/g and oven-dried culture with tapioca filler was 8,94 log CFU/g. The viability of oven-dried L. plantarum HL-15 decreased during two-month storage.
Keywords
viability, antifungal, oven-dried culture, lactobacillus plantarum HL 15
References
2 Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia
watipasca2@gmail.com
Abstract
Lactobacillus plantarum HL-15 has the ability to inhibit the growth of mycotoxin-producing fungi. To support the application of the use of these cultures, a study was carried out aimed at producing dry L. plantarum HL-15 and observing its stability during storage. Production of dry culture begins with fermentation of L. plantarum HL-15 then centrifugation is carried out to obtain pellets. Pellets were mixed with fillers (rice flour or tapioca) with a ratio of pellets: fillers (10%) = 1: 1 (v / v) and then dried. The drying machine used is an oven with a temperature of 45 ° C for 20 hours. The dry culture was packaged in aluminum foil and sealed and then stored at 4 ° C. The result showed that viable cells of oven-dried L. plantarum HL-15 with rice filler was <4log CFU/g and oven-dried culture with tapioca filler was 8,94 log CFU/g. The viability of oven-dried L. plantarum HL-15 decreased during two-month storage.
